Which immersion oil to use

Oil left on lens surfaces will eventually dry and will be very difficult to remove. The quality and resolution of your image depends mainly on the Numerical Aperture NA of used objective. To very briefly, the NA relates to the light gathering properties of the optical components of your microscope.

Using an immersion lens and oil can improve both your resolution and NA. Most microscope coverslips will have refractive indexes of 1. Therefore, if you use a dry objective the light rays from your specimen will undergo refraction when travelling from the glass coverslip into the air.

Developed low fluorescence immersion oil First to print key optical properties on every bottle First to print full tables of optical and physical properties in Data Sheets Department of Defense. Developed the first very high viscosity oils for horizontal, inverted and projecting microscopes in cooperation with US Department of H. Developed the first PCB-free immersion oils Supplies oil to meet British immersion oil specifications.

Collaborated with DIN and ISO committees in developing international standards and served as liaison with American microscope manufacturers.

Provides modified formulas for special applications supported by Extended Properties Technical Bulletins. First to modify immersion oil formulations for use with various instruments such as calibration, mounting, contact and interface fluids in the fields of fiber optics, lasers, optometry, and optical engineering where special index, temperature, wavelength, viscosity, density and adsorption characteristics are required.

First in worldwide distribution of immersion oils. Download KB. Objective Selector. Overview Specifications Immersion oil increases the resolving power of the microscope by replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction. Type N Type N is a general purpose oil recommended for transmitted light applications, including brightfield, darkfield, DIC and phase contrast.

Type F Type F is designed to ensure the highest image quality for fluorescence applications, with maximal signal-to-noise ratio and minimal autofluorescence. Silicone Silicone immersion oil is used for deep observations of live specimens.